Healthcare

Biography

Sergey Goncharov  has been Graduated from Biological and Postgraduate faculties in Gomel State University (1999) with the specialties Biology, Chemistry and Practical Psychology, where later obtained Diploma in Course of the Polish Language (2011). Then started working at the Institute of Radiobiology of the National Academy of Sciences of Belarus where he has continued his education (2007-2010 – Aspirant in the specialty Radiobiology). During 2010 – Holder of Presidential Scholarship. Since 2011 as a Researcher in Laboratory of Combined Effects has been a responsible executive in 4 scientific projects. Presently he works in the field of Photoprotection and Waste Management in Gomel.

 

Abstract

Studying dry extracts of Hypogymnia physodes, one of the most common lichens in Belarus, aimed to investigate its toxicity and UV-protective properties in model assays in vivo and on cell cultures. Made on ethanol, acetone and hexane-acetone, the extracts effectively absorb the most biologically active range of UV (250-400 nm).

In vivo (on mice Af) an efficiency of the extracts (5% solutions) in dimethilsulfoxide was comprehensively assessed. On the 4th day after 30 min exposing skin to UV-A/B we observed brown skin burns, scab and 50% thicker skin; in serum – significant increase of prooxidant capacity, advanced oxidation protein products (AOPP), nitrate/nitrite ions (Nox), glutathione peroxidase activity (GPx), reduced levels of glutathione and protein SH-groups.

In the same conditions, single topical applications of all the extracts decreased GPx, prooxidant capacity (24-40%), AOPP (9-20%) and Nox (27-42%, except ethanol extract) with simultaneous increase of protein SH-groups (62-98%), compared to UV-control. Acetone and hexane-acetone extracts reduced scab, edema and inflammation on skin and have shown the best photoprotection.