Healthcare

Biography

Dr.Rostand Idriss TCHANA TCHAMBA Graduated from the University of Lubumbafi (UNILU) as a Pharmacist, with the speciality including Basic Heatlh care and drugs management, Clinical biology. He also trained in experimental pharmacology research at the Malagasy Institute of applied Reserch (IMRA) in Madagasgar where he featured in different pharmacology studies on plants extracts. Now he’s back in Cameroon and works at the Regional Hospital of Maroua in the far north region and continues his research in partnership with the University of Lubumbashi.

 

Abstract

Malaria remains a major public health concern in Sub-Saharan Africa, particularly in DRC (Democratic republic of Congo). Dialium Angolensis (Malais) DC (Fabaceae) called Irhuza or Ituza by local tribes, is a plant widely used to cure various pathologies including infections and malaria in traditional medicine. We evaluated in vivo the antiplasmodial activity of various extracts of 200 mg / kg plant materials derived from Dialium Angolensis. We assessed the microbiological and bacteriological activities, the acute toxicity and identified the various phytochemical groups present in this plant.

 

            The phytochemical screening was carried out by conventional physicochemical methods on the extracts of the plant material. The 4-days suppressive test described by Peters et al. in 2004 was carried out on different ethanolic extracts of leaves, aqueous extracts of leaves, ethanolic extracts barks and stems, and dechlorophylled ethanolic extracts of leaves; Using SWISS mices with the inoculum of red cells parasitized by Plasmodium yoelii. The parasitaemia of treated elements was determined by microscopic reading of blood smears (blood from the tail) and the percentage of chemosuppression of the parasitemia calculated as a function of the negative control group.

 

The phytochemical screening demonstrated the presence of at least four bioactive chemical groups on the screened vegetative organs. The evaluation of the antimalarial activity (in vivo) showed an average inhibition rate of 54.39% for the most active extract. The microbiological tests showed a bactericidal activity on staphylococci and streptococci. The acute toxicity test revealed that Dialium angolense may be a non-toxic based on the Sterner and Hodge scale.